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KMID : 1134820210500040322
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Journal of the Korean Society of Food Science and Nutrition
2021 Volume.50 No. 4 p.322 ~ p.329
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The Effect of Chaenomelis Fructus on Thioacetamide-Induced Acute Hepatic Injury
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Lee Jin-A
Shin Mi-Rae
Lee Ji-Hye
Park Hae-Jin
Roh Seong-Soo
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Abstract
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This study investigated the effect of Chaenomelis Fructus extract (CF) on thioacetamide (TAA)-induced acute liver injury in rats. Rats were divided into five treatment groups: Normal group (Normal, n=8), TAA-induced with distilled water group (Control, n=8), TAA-induced with silymarin 100 mg/kg group (Silymarin, n=8), TAA-induced with CF 100 mg/kg group (CF100, n=8), and TAA-induced with CF 200 mg/kg group (CF200, n=8). An animal model of acute liver injury was established via an injection of TAA (200 mg/kg of body weight, I.P). Silymarin and CF extracts were administrated (P.O.) simultaneously with TAA for 3 days. Levels of GOT (glutamic oxaloacetic transaminase), GPT (glutamic pyruvic transaminase), ammonia, and MPO (myeloperoxidase) were assessed in serum, and the expressions of NADPH oxidase and inflammatory related proteins were analyzed by western blot from liver tissue harvested during autopsy. Our results reveal that CF-administration significantly decreases the levels of serum GOT, GPT, ammonia, and MPO, effectively suppresses NADPH oxidase, and down-regulates the expressions of the NF-κB pathway proteins. These results indicate that administration of CF alleviates the liver injury by inhibiting NADPH oxidase and down-regulating the NF-κB pathway.
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KEYWORD
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Chaenomelis Fructus, acute hepatic injury, thioacetamide, anti-inflammation
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